Problematic barcoding in flatworms: A case-study on monogeneans and rhabdocoels (Platyhelminthes)

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Publikace nespadá pod Ústav výpočetní techniky, ale pod Přírodovědeckou fakultu. Oficiální stránka publikace je na webu muni.cz.
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VANHOVE Maarten Pieterjan TESSENS Bart SCHOELINCK Charlotte JONDELIUS Ulf LITTLEWOOD D. Tim J. ARTOIS Tom HUYSE Tine

Rok publikování 2013
Druh Článek v odborném periodiku
Časopis / Zdroj ZOOKEYS
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
Doi http://dx.doi.org/10.3897/zookeys.365.5776
Obor Zoologie
Klíčová slova mitochondrial DNA; Monogenea; primer design; ribosomal DNA; Rhabdocoela; turbellarians
Popis Some taxonomic groups are less amenable to mitochondrial DNA barcoding than others. Due to the paucity of molecular information of understudied groups and the huge molecular diversity within flatworms, primer design has been hampered. Indeed, all attempts to develop universal flatworm-specific COI markers have failed so far. We demonstrate how high molecular variability and contamination problems limit the possibilities for barcoding using standard COI-based protocols in flatworms. As a consequence, molecular identification methods often rely on other widely applicable markers. In the case of Monogenea, a very diverse group of platyhelminth parasites, and Rhabdocoela, representing one-fourth of all free-living flatworm taxa, this has led to a relatively high availability of nuclear ITS and 18S/28S rDNA sequences on GenBank. In a comparison of the effectiveness in species assignment we conclude that mitochondrial and nuclear ribosomal markers perform equally well. In case intraspecific information is needed, rDNA sequences can guide the selection of the appropriate (i.e. taxon-specific) COI primers if available.
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