Reaction-based Indicator displacement Assay (RIA) for the selective colorimetric and fluorometric detection of peroxynitrite

Varování

Publikace nespadá pod Ústav výpočetní techniky, ale pod Středoevropský technologický institut. Oficiální stránka publikace je na webu muni.cz.

Autoři	SUN Xiaolong LACINA Karel RAMSAMY Elena C. FLOWER Stephen E. FOSSEY John S. QIAN Xuhong ANSLYN Eric V. BULL Steven D. JAMES Tony D.
Rok publikování	2015
Druh	Článek v odborném periodiku
Časopis / Zdroj	CHEMICAL SCIENCE
Fakulta / Pracoviště MU	Středoevropský technologický institut
Citace
www	http://pubs.rsc.org/en/content/articlelanding/2015/sc/c4sc03983a#!divAbstract
Doi	https://doi.org/10.1039/c4sc03983a
Obor	Analytická chemie, separace
Klíčová slova	APPENDED BIPYRIDINIUM SALTS; BORONIC ACID; HYDROGEN-PEROXIDE; NITRIC-OXIDE; FLUORESCENT-PROBES; SENSING SYSTEM; GLUCOSE; CHEMOSENSOR; REPORTER; VIOLOGEN
Popis	Using the self-assembly of aromatic boronic acids with Alizarin Red S (ARS), we developed a new chemosensor for the selective detection of peroxynitrite. Phenylboronic acid (PBA), benzoboroxole (BBA) and 2-(N,N-dimethylaminomethyl)phenylboronic acid (NBA) were employed to bind with ARS to form the complex probes. In particular, the ARS–NBA system with a high binding affinity can preferably react with peroxynitrite over hydrogen peroxide and other ROS/RNS due to the protection of the boron via the solvent-insertion B–N interaction. Our simple system produces a visible colorimetric change and on–off fluorescence response towards peroxynitrite. By coupling a chemical reaction that leads to an indicator displacement, we have developed a new sensing strategy, referred to herein as RIA (Reactionbased Indicator displacement Assay).
Související projekty:	CEITEC - středoevropský technologický institut