Off-line CE-MALDI MS/SALD ICP MS coupling: what can we acquire from a single separation run?
| Autoři | |
|---|---|
| Rok publikování | 2014 |
| Druh | Další prezentace na konferencích |
| Fakulta / Pracoviště MU | |
| Citace | |
| Popis | Single run of CE coupled to MALDI MS and SALD-ICP MS provides highly complementary information about present biomolecules and metals. Moreover, SALD-ICP MS allows reliable metal quantitation, which was performed using a calibration curve approach. Both protein apoforms and protein-metal complexes can be detected using MALDI MS, depending on the applied MALDI matrix and the pH of the matrix solution. MALDI mass spectra of metal-protein complexes are compared with corresponding ESI mass spectra. |
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