Quantification of Golgi Complex Assembly and Disassembly in LiveCell Fluorescence Microscopy Images

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MATULA Petr LISAUSKAS Tautvydas STARKUVIENE Vytaute ROHR Karl

Rok publikování 2011
Druh Článek ve sborníku
Konference Proceedings of Workshop on Microscopic Image Analysis with Applications in Biology
Fakulta / Pracoviště MU

Fakulta informatiky

Citace
www http://www.miaab.org/miaab-2011-heidelberg-papers/miaab-2011-h-matula.pdf
Obor Informatika
Klíčová slova Fluorescence microscopy; quantification of subcellular localization patterns; tophat transform; Golgi assembly and disassembly; compact and diffuse phenotypes
Popis We present an image analysis approach for quantifying assembly and disassembly of the Golgi complex in fluorescence microscopy images of live cells. The approach comprises nucleus detection, nucleus tracking, and quantification of Golgi complex localization patterns for single cell nuclei over time. In particular, we define and compare five different measures for quantifying changes in compact and diffuse phenotypes. We propose two measures based on mathematical morphology which take into account shape, size, and contrast of image structures. From the experimental results it turned out that these measures have a higher discrimination power to distinguish compact and diffuse phenotypes than a previous frequently used measure.
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