WAVELENGTH AND CROSSLINKER EFFECTS ON GELATIN METHACRYLATE HYDROGEL PHOTOPOLYMERIZATION
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| Rok publikování | 2024 |
| Druh | Další prezentace na konferencích |
| Citace | |
| Popis | Abstract Hydrogels based on gelatin methacrylate (Gel-MA) have garnered significant attention in tissue engineering and regenerative medicine due to their excellent biocompatibility and tunable properties. However, Gel-MA alone does not form a hydrogel, necessitating the use of different crosslinkers. Photopolymerization is one method utilized to create a rigid structure. In this experiment, we investigated the influence of 365 nm and 405 nm wavelengths on hydrogel curing time using various concentrations of 2-Hydroxy-4'-(2-hydroxyethoxy)-2-methylpropiophenone (I2959) and lithiumphenyl-2,4,6-trimethylbenzoylphosphinate (LAP). Different concentrations of I2959 (0.05%, 0.2%, 0.5%, and 1% w/v) and LAP (0.05%, and 1% w/v) were added to the Gel-MA solution to form hydrogels. Curing was carried out using UV light sources emitting at 365 nm and 405 nm, with exposure times ranging from 5 seconds to 15 minutes. All hydrogels prepared with different concentrations of I2959, and LAP successfully cured under 365 nm UV light. The fastest curing time achieved with I2959 was 3 minutes, whereas LAP demonstrated more efficient curing with a shorter exposure time of 10 seconds. In contrast, under 405 nm UV light, I2959 failed to achieve successful curing, while LAP showed poor results. Spectrophotometric analysis of both curing agents supported these findings |
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