Inflammatory reaction of the choroid plexus induced by the diabetes mellitus
| Autoři | |
|---|---|
| Rok publikování | 2025 |
| Druh | Další prezentace na konferencích |
| Fakulta / Pracoviště MU | |
| Citace | |
| Popis | Diabetes mellitus (DM) is chronic metabolic disease affected, without exception, the whole organism including the central nervous system. One of the lesser-explored diabetic complication is diabetic encephalopathy (DE), where structural and functional alterations of the brain are evident. Pathophysiology of the DE is complex and there is certainly involvement of the choroid plexus as the blood-cerebrospinal fluid barrier (BCSFB) due to its immunological and barrier functions. In this study, we investigated the inflammatory response of the choroid plexus in a rat model of type 1 DM. Male Wistar rats (8–10-week-old males) were divided into two groups: the diabetic group (n=13) and the control group (n=8). DM was induced by a single intraperitoneal injection of streptozotocin (80 mg/kg), while controls received only vehicle. After three weeks, animals were euthanized using CO2 inhalation, followed by perfusion with Zamboni’s fixative. Brains were collected and processed for coronal sectioning. Immunohistochemical staining of the lateral ventricular choroid plexus was performed using markers of macrophage/microglial activation and polarization: CD68, CD163, CCR7, CD206, CD11b/c, and MHC-II. The number of positive cells per mm2 was examined and statistically evaluated. Our results demonstrate a significant increase in immune cell infiltration and activation in the choroid plexus of diabetic rats compared to controls. These findings support the hypothesis that inflammatory response of the choroid plexus induced by DM could lead to disruption of brain microenvironment and development of the DE. Therefore, the choroid plexus may represent a potential therapeutic target for modulating neuroinflammation in diabetes. |
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