Comprehensive profiling of lncRNAs and mRNAs enriched in small extracellular vesicles for early noninvasive detection of colorectal cancer: diagnostic panel assembly and extensive validation

Varování

Publikace nespadá pod Ústav výpočetní techniky, ale pod Lékařskou fakultu. Oficiální stránka publikace je na webu muni.cz.

Autoři	VYCHYTILOVÁ Petra PAVLÍKOVÁ Markéta PIFKOVÁ Lucie JUGAS Robin MACHÁČKOVÁ Táňa RADOVÁ Lenka SACHLOVA Milana BARTOŠOVÁ Renata SAMOILENKO Tetiana FEČKOVÁ Zuzana ORLÍČKOVÁ Jana SLÁMOVÁ Erika PONECHAL MICHU Elleni AL TUKMACHI Dagmar RUČKOVÁ Michaela VODINSKÁ Martina KOTOUCEK Jan CATELA IVKOVIĆ Tina BOUDNÁ Marie BOHOVICOVÁ Lucia STANĚK Teodor HALÁMKOVÁ Jana SVOBODA Martin PROCHÁZKA Vladimír GROLICH Tomáš KALA Zdeněk SLABÝ Ondřej
Rok publikování	2025
Druh	Článek v odborném periodiku
Časopis / Zdroj	MOLECULAR ONCOLOGY
Fakulta / Pracoviště MU	Lékařská fakulta
Citace
www	https://febs.onlinelibrary.wiley.com/doi/10.1002/1878-0261.70086
Doi	https://doi.org/10.1002/1878-0261.70086
Klíčová slova	colorectal cancer; diagnostic panel; high-throughput expression profiling; long noncoding RNA; small extracellular vesicle
Přiložené soubory	Comprehensive_profiling_of_lncRNAs_and_mRNAs_enriched_in_small_extracellular_vesicles_for_early_noninvasive_detection_of_colorectal_cancer_diagnostic_panel_assembly_and_extensive_validation.pdf
Popis	Early diagnosis of colorectal cancer (CRC) is crucial for successful treatment and mortality reduction. In this regard, blood-based tests play an indispensable role. Current research is focused on molecules actively secreted by tumor cells into small extracellular vesicles (EVs). This four-phase study included 613 CRC patients, 446 controls, and 120 precancerous lesions. High-throughput transcriptome profiling of small EVs was performed on samples from 100 CRC patients and 50 controls, followed by extensive validation using reverse transcription quantitative polymerase chain reaction. Diagnostic panels were developed via logistic regression and further characterized by enrolling samples from gastric cancer patients, CRC patients before/after surgery, and samples of tumor tissues/adjacent mucosa. We identified 17 molecules significantly elevated in CRC, with the highest levels in metastatic cases. Additionally, seven of them differentiated controls from precancerous lesions. Two diagnostic panels were developed, enabling early CRC detection with high sensitivity and specificity, outperforming the fecal occult blood test. Furthermore, six molecules were differentially expressed between tumor tissue and mucosa, while seven EV-enriched molecules decreased significantly after surgery. These findings highlight EVs as key reservoirs of CRC-associated molecules and a promising source of biomarkers.
Související projekty:	Dlouhé nekódující RNA v exozómech jako biomarkery umožňující neinvazivní časnou detekci kolorektálního karcinomu Národní ústav pro výzkum rakoviny