Irreversible furin cleavage site exposure renders immature tick-borne flaviviruses fully infectious

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Publikace nespadá pod Ústav výpočetní techniky, ale pod Přírodovědeckou fakultu. Oficiální stránka publikace je na webu muni.cz.
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HOLOUBEK Jiří SALÁT Jiří MATKOVIC Milos BEDNÁŘ Petr NOVOTNÝ Pavel HRADILEK Martin MAJEROVÁ Taťána ROSENDAL Ebba EYER Luděk FOŘTOVÁ Andrea BERÁNKOVÁ Michaela BELL-SAKYI Lesley ÖVERBY Anna K CAVALLI Andrea BONOMI Massimiliano REY Felix A RŮŽEK Daniel

Rok publikování 2025
Druh Článek v odborném periodiku
Časopis / Zdroj Nature Communications
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
www https://www.nature.com/articles/s41467-025-62750-6
Doi https://doi.org/10.1038/s41467-025-62750-6
Klíčová slova Viral pathogenesis; Virus–host interactions
Přiložené soubory
Popis Flavivirus assembly is driven by the envelope glycoproteins pre-membrane (prM) and envelope (E) in the neutral pH environment of the endoplasmic reticulum. Newly budded, spiky particles are exported through the Golgi apparatus, where mildly acidic pH induces a major surface rearrangement. The glycoproteins reorganize into (prM/E)\2 complexes at the surface of smooth particles, with prM trapped at the E dimer interface, thereby exposing a furin cleavage site (FCS) for proteolytic maturation into infectious virions. Here, we show that in the absence of furin, immature tick-borne flavivirus particles—tick-borne encephalitis virus, Langat virus, and Louping ill virus—remain fully infectious and pathogenic in female BALB/c mice, in contrast to mosquito-borne flaviviruses such as Usutu, West Nile, and Zika viruses. We further show that the FCS in tick-borne viruses remains exposed at neutral pH, allowing furin at the surface of target cells to activate viral fusogenicity, while mosquito-borne counterparts require acidic re-exposure. Mutations increasing the dynamic behavior of the E dimer mimic the mosquito-borne phenotype, with retracted FCS at neutral pH and loss of infectivity. Our multidisciplinary approach—combining virological assays, targeted mutagenesis, structural modeling, and molecular dynamics simulations—highlights the role of E dimer dynamics in regulating flavivirus maturation and infectivity.
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