Plasma mediated immobilization of metformin on polyethylene: effects on drug release, antibacterial activity, and biocompatibility

Varování

Publikace nespadá pod Ústav výpočetní techniky, ale pod Přírodovědeckou fakultu. Oficiální stránka publikace je na webu muni.cz.
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ZIDEK Stepan STEPANKOVA Katerina PISTEKOVA Hana MASAR Milan STUPAVSKÁ Monika SŤAHEL Pavel TRUNEC David MOZETIC Miran VALASEK Pavel LEHOCKY Marian

Rok publikování 2025
Druh Článek v odborném periodiku
Časopis / Zdroj JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
Fakulta / Pracoviště MU

Přírodovědecká fakulta

Citace
www https://www.tandfonline.com/doi/full/10.1080/09205063.2025.2524261
Doi http://dx.doi.org/10.1080/09205063.2025.2524261
Klíčová slova Antibacterial coating; surface modification; plasma treatment; metformin; diabetes
Popis Metformin, a widely used antidiabetic drug, has gained attention for its potential applications in antimicrobial surfaces, delivery systems, and anticancer therapy. However, immobilizing metformin in a stable, bioactive, and dose-controllable manner onto a chemically inert, hydrophobic surface is challenging. The objective of this study is to immobilize metformin at various concentration (0.5, 1, 2, 5, 10, and 20 gL-1) onto low-density polyethylene (LDPE) surfaces by a multistep approach with the aim of creating bioactive coatings. In this approach, LDPE was first treated with a 40 kHz low pressure plasma discharge in air atmosphere, followed by non-covalent attachment of acrylic acid via a grafting technique. Metformin was covalently attached to the surface via N-(3-Dimethylaminopropyl)-N '-ethylcarbodiimide hydrochloride (EDC) and N-Hydroxysuccinimide (NHS) activation, while its presence on the polymer surface was confirmed by Water contact angle (WCA), Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM). Sustained metformin release with a shift from Fickian to first-order kinetics was observed at higher drug loading. Antibacterial testing against Staphylococcus aureus and Escherichia coli showed no antibacterial effect at the selected concentration levels. Cytocompatibility assays with multipotent mesenchymal cells showed good biocompatibility of modified surfaces, with only dose-dependent cytotoxicity at higher metformin concentrations (>5 gL-1). These results demonstrate that despite the absence of antibacterial effects, the developed system offers a promising platform for further biomedical applications requiring controlled drug surface functionalization and retained cytocompatibility.
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