Synthesis of Tetravalent Thio- and Selenogalactoside-Presenting Galactoclusters and Their Interactions with Bacterial Lectin PA-IL from Pseudomonas aeruginosa

Varování

Publikace nespadá pod Ústav výpočetní techniky, ale pod Přírodovědeckou fakultu. Oficiální stránka publikace je na webu muni.cz.

Autoři	ILLYÉS Tünde Zita MALINOVSKÁ Lenka RŐTH Erzsébet TÓTH Boglárka FARKAS Bence KORSÁK Marek WIMMEROVÁ Michaela KÖVÉR Katalin E. CSÁVÁS Magdolna
Rok publikování	2021
Druh	Článek v odborném periodiku
Časopis / Zdroj	Molecules
Fakulta / Pracoviště MU	Přírodovědecká fakulta
Citace
www	https://doi.org/10.3390/molecules26030542
Doi	https://doi.org/10.3390/molecules26030542
Klíčová slova	selenoglycosides; galactoclusters; Pseudomonas aeruginosa; PA-IL lectin; multivalency
Popis	Synthesis of tetravalent thio- and selenogalactopyranoside-containing glycoclusters using azide-alkyne click strategy is presented. Prepared compounds are potential ligands of Pseudomonas aeruginosa lectin PA-IL. P. aeruginosa is an opportunistic human pathogen associated with cystic fibrosis, and PA-IL is one of its virulence factors. The interactions of PA-IL and tetravalent glycoconjugates were investigated using hemagglutination inhibition assay and compared with mono- and divalent galactosides (propargyl 1-thio- and 1-seleno-beta-d-galactopyranoside, digalactosyl diselenide and digalactosyl disulfide). The lectin-carbohydrate interactions were also studied by saturation transfer difference NMR technique. Both thio- and seleno-tetravalent glycoconjugates were able to inhibit PA-IL significantly better than simple d-galactose or their intermediate compounds from the synthesis.
Související projekty:	Lektiny a jejich úloha v interakci patogen/hostitel a buněčném rozpoznávání Česká národní infrastruktura pro biologická data