Integration of Cryo-EM Model Building Software in Scipion
| Autoři | |
|---|---|
| Rok publikování | 2020 |
| Druh | Článek v odborném periodiku |
| Časopis / Zdroj | Journal of Chemical Information and Modeling |
| Fakulta / Pracoviště MU | |
| Citace | |
| www | http://dx.doi.org/10.1021/acs.jcim.9b01032 |
| Doi | http://dx.doi.org/10.1021/acs.jcim.9b01032 |
| Klíčová slova | ELECTRON-MICROSCOPY; LOCAL RESOLUTION; VALIDATION TOOLS; VISUALIZATION; MAPS |
| Popis | Advances in cryo-electron microscopy (cryo-EM) have made it possible to obtain structures of large biological macromolecules at near-atomic resolution. This "resolution revolution" has encouraged the use and development of modeling tools able to produce high-quality atomic models from cryo-EM density maps. Unfortunately, many practical problems appear when combining different packages in the same processing workflow, which make difficult the use of these tools by non-experts and, therefore, reduce their utility. We present here a major extension of the image processing framework Scipion that provides inter-package integration in the model building area and full tracking of the complete workflow, from image processing to structure validation. |
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