Analysis of microorganisms, chlorinated hydrocarbons and hyaluronic acid gel using Raman based optofluidic techniques and SERS

• Autoři: SAMEK Ota; PILAT Zdenek; BERNATOVÁ Silvie; JEZEK Jan; KLEMENTOVA Tereza; KIZOVSKÝ Martin; ZEMANEK Pavel; REBROŠOVÁ Katarína; RŮŽIČKA Filip
• Rok publikování: 2019
• Druh: Článek ve sborníku
• Konference: SPIE BiOS, 2019, San Francisco, California, United States
• Fakulta / Pracoviště MU: Lékařská fakulta
• Doi: https://doi.org/10.1117/12.2508425
• Klíčová slova: Raman spectroscopy - SERS
• Popis: We report on the development of a set of Raman based techniques to monitor a large variety of biological and chemical analytes, such as various microorganisms, gels of hyaluronic acid and selected halogenated hydrocarbons using Raman spectroscopy, Raman tweezers and surface-enhanced Raman spectroscopy (SERS). We analyzed individual microbial cells with Raman tweezers to provide solutions for fast and label-free identification of specific bacterial or yeast species. We designed an optofluidic SERS platform for quantification of sub-millimolar concentrations of halogenated environmental pollutants such as 1,2,3-trichloropropane and chloroform. We also examined the gel structure of hyaluronic acid by Raman spectroscopy.

Související projekty

• Původci nozokomiálních nákaz a možnosti jejich diagnostiky a terapie