Allelic variants for candidate nitrogen fixation genes revealed by sequencing in red clover (Trifolium pratense L.)

Investor logo

Warning

This publication doesn't include Institute of Computer Science. It includes Faculty of Science. Official publication website can be found on muni.cz.
Authors

TRNĚNÝ Oldřich VLK David MACKOVÁ Eliška MATOUŠKOVÁ Michaela ŘEPKOVÁ Jana NEDĚLNÍK Jan HOFBAUER Jan VEJRAŽKA Karel JAKEŠOVÁ Hana JANSA Jan PIÁLEK Lubomír KNOTOVÁ Daniela

Year of publication 2019
Type Article in Periodical
Magazine / Source International Journal of Molecular Sciences
MU Faculty or unit

Faculty of Science

Citation
Web http://dx.doi.org/10.3390/ijms20215470
Doi http://dx.doi.org/10.3390/ijms20215470
Keywords associated genes; associated polymorphisms; genome-wide association; biological nitrogen fixation; red clover
Description Plant–rhizobia symbiosis can activate key genes involved in regulating nodulation associated with biological nitrogen fixation (BNF). Although the general molecular basis of the BNF process is frequently studied, little is known about its intraspecific variability and the characteristics of its allelic variants. This study’s main goals were to describe phenotypic and genotypic variation in the context of nitrogen fixation in red clover (Trifolium pratense L.) and identify variants in BNF candidate genes associated with BNF efficiency. Acetylene reduction assay validation was the criterion for selecting individual plants with particular BNF rates. Sequences in 86 key candidate genes were obtained by hybridization-based sequence capture target enrichment of plants with alternative phenotypes for nitrogen fixation. Two genes associated with BNF were identified: ethylene response factor required for nodule differentiation (EFD) and molybdate transporter 1 (MOT1). In addition, whole-genome population genotyping by double-digest restriction-site-associated sequencing (ddRADseq) was performed, and BNF was evaluated by the natural 15N abundance method. Polymorphisms associated with BNF and reflecting phenotype variability were identified. The genetic structure of plant accessions was not linked to BNF rate of measured plants. Knowledge of the genetic variation within BNF candidate genes and the characteristics of genetic variants will be beneficial in molecular diagnostics and breeding of red clover.
Related projects:

You are running an old browser version. We recommend updating your browser to its latest version.

More info