Cytokinins control secondary cell wall formation in the inflorescence stem of Arabidopsis
| Authors | |
|---|---|
| Year of publication | 2025 |
| Type | Article in Periodical |
| MU Faculty or unit | |
| Citation | |
| web | https://is.muni.cz/publication/2524423 |
| Doi | https://doi.org/10.5817/IS.MUNI.CZ2025-2522180 |
| Keywords | cytokinins; multistep phosphorelay; NAC TFs; tracheary elements; interfascicular fibers; Arabidopsis |
| Attached files | |
| Description | Spatiotemporal control over developmental programs is vital to all organisms. Here we show that deficiency in cytokinin signaling or biosynthesis leads to early secondary cell wall (SCW) formation in Arabidopsis inflorescence stem that associates with precocious upregulation of a SCW transcriptional cascade controlled by NAC TFs (NSTs/VNDs). Cytokinin signaling through the AHK2/3 and the ARR1/10/12 suppresses the expression of several NSTs and SCW formation. Exogenous cytokinin application led to fast downregulation of NST1 and NST3 (NST1/3) in the wild type (WT) and reconstituted both proper development and apical-basal gradient of NST1/3 expression in a cytokinin-deficient mutant. AHK2 and AHK3 require functional NST1 or NST3 to control SCW initiation in the interfascicular fibers, evidencing that cytokinins act upstream of NSTs. The premature onset of a rigid SCW biosynthesis and altered expression of NSTs due to cytokinin deficiency led to the formation of smaller tracheary elements (TEs) and impaired hydraulic conductivity. We conclude that cytokinins downregulate NSTs to inhibit premature SCW formation in the apical part of the inflorescence stem, facilitating thus the development of fully functional TEs and interfascicular fibers. Fully published version of the this article is available https://is.muni.cz/auth/publication/2524423 |
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