Haloalkane dehalogenases other than LinB can contribute to the γ-hexachlorocyclohexane utilization
| Authors | |
|---|---|
| Year of publication | 2025 |
| Type | Article in Periodical |
| Magazine / Source | BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY |
| MU Faculty or unit | |
| Citation | |
| web | https://academic.oup.com/bbb/article-abstract/89/6/898/8052007?redirectedFrom=fulltext&login=false |
| Doi | https://doi.org/10.1093/bbb/zbaf022 |
| Keywords | haloalkane dehalogenase; xenobiotics; pesticide; gamma-hexachlorocyclohexane; sphingomonads |
| Description | Haloalkane dehalogenases (HLDs) convert halogenated compounds to corresponding alcohols by a simple hydrolytic mechanism. Although many bacterial strains possess HLDs or HLD homologues, LinB is the only HLD known to be involved in the utilization of gamma-hexachlorocyclohexane (gamma-HCH), a man-made chlorinated pesticide. In this study, to gain insight into the functional evolution of HLDs toward gamma-HCH utilization, the linB gene in gamma-HCH-degrading Sphingobium japonicum strain UT26 was replaced by 7 other HLD or HLD homologous genes, including 3 putative ancestral enzymes. Interestingly, strains carrying genes for DmmA from a marine metagenome or Rluc_anc, an ancestor of Renilla-luciferin 2-monooxygenase and LinB, produced 2,5-dichlorophenol and 2,5-dichlorohydroquinone from gamma-HCH, which are indicators of LinB activity, and grew in minimal medium supplied with gamma-HCH as a sole carbon source. These results indicated that other HLDs, in addition to LinB, can play roles in the gamma-HCH utilization. |
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