Eudiplozoon nipponicum (Heteronchoinea: Diplozoidae): insight into composition of E/S products and basic characterization of serpin

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Authors	ROUDNICKÝ Pavel VOREL Jiří ILGOVÁ Jana MIKEŠ Libor JEDLIČKOVÁ Lucie DALTON John DVOŘÁK Jan ZDRÁHAL Zbyněk POTĚŠIL David JANDA Lubomír NOREK Adam GELNAR Milan KAŠNÝ Martin
Year of publication	2016
Type	Appeared in Conference without Proceedings
MU Faculty or unit	Faculty of Science
Citation
Description	Two genes coding serpins, were expressed in E. coli system (BL21 strain) in combination with synthetic genes, optimised for this system, using pET-22B(+) expression vector. IPTG induced expression (30°C, overnight) was analyzed on SDS-PAGE and soluble recombinant serpins of E. nipponicum was partially purified using Ni-NTA agarose beads. Successful production of serpins was verified by mass spectrometry and Western blot using His-tag primary antibodies.
Related projects:	ECIP - Evropské centrum ichtyoparazitologie