Screening a cDNA Library for Protein-Protein Interactions Directly in Planta

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Authors	LEE Lan-Ying WU Fu-Hui HSU Chen-Tran SHEN Shu-Chen YEH Hsuan-Yu LIAO De-Chih FANG Mei-Jane LIU Nien-Tze YEN Yu-Chen DOKLÁDAL Ladislav SÝKOROVÁ Eva GELVIN Stanton B. LIN Choun-Sea
Year of publication	2012
Type	Article in Periodical
Magazine / Source	Plant Cell
MU Faculty or unit	Central European Institute of Technology
Citation
web	http://www.plantcell.org/content/24/5/1746.abstract
Doi	https://doi.org/10.1105/tpc.112.097998
Field	Genetics and molecular biology
Keywords	BIMOLECULAR FLUORESCENCE COMPLEMENTATION; STRAND TELOMERIC DNA; BINDING-PROTEIN; NUCLEAR IMPORT; IN-PLANTA; T-DNA; VIRD2 PROTEIN; CELLS; TRANSFORMATION; VISUALIZATION
Description	Screening cDNA libraries for genes encoding proteins that interact with a bait protein is usually performed in yeast. However, subcellular compartmentation and protein modification may differ in yeast and plant cells, resulting in misidentification of protein partners. T-DNA insertions in the genes represented by some cDNAs revealed five novel Arabidopsis proteins important for Agrobacterium-mediated plant transformation. We also used this cDNA library to confirm VirE2-interacting proteins in orchid (Phalaenopsis amabilis) flowers. Thus, this technology can be applied to several plant species.
Related projects:	Telomeres and telomerase: transition from molecular to structural biology approach