Identification and determination of relatedness of lactobacilli using different DNA amplification methods

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Authors

TURKOVÁ Kristýna RITTICH Bohuslav ŠPANOVÁ Alena

Year of publication 2012
Type Article in Periodical
Magazine / Source Chemical Papers
MU Faculty or unit

Faculty of Science

Citation
Doi http://dx.doi.org/10.2478/s11696-012-0206-7
Field Genetics and molecular biology
Keywords Lactobacillus; breast fed children; dairy products; identification; PCR; fingerprinting
Description Several DNA amplification-based methods were used for identification and evaluation of the relation between lactobacilli isolated from breastfed full-term infant faeces, dairy products and silage. Twenty-seven strains isolated friom infant faeces were identified as Lactobacillus rhamnosus, Lactobacillus gasseri, Lactobacillus paracasei, Lactobacillus fermentum, Lactobacillus salivarius, Lactobacillus plantarum, and Lactobacillus helveticus using ten species-specific PCRs, multiplex PCR, and sequencing of 16SrDNA. Four strains were not identified. A repetitive extragenic palindromic PCR (rep-PCR) and randomly amplified polymorphic DNA PCR (RAPD-PCR) were used for confirmation of species identification. Fingerprints were used for evaluation of the relatedness of lactobacilli. Differences between strains from infant faeces, dairy products, and silage were not detected.
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