Electrophoretic techniques in the analysis of polydeoxycytidylic acids

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Authors	BITTOVÁ Miroslava JELÍNKOVÁ Kateřina ROBLOVÁ Vendula TRNKOVÁ Libuše
Year of publication	2012
Type	Article in Proceedings
Conference	XII. Pracovní setkání fyzikálních chemiků a elektrochemiků. Sborník příspěvků
MU Faculty or unit	Faculty of Science
Citation
Field	Analytic chemistry
Keywords	electrophoresis oligonucleotides coated capillary
Description	Electrophoretic techniques such as capillary zone electrophoresis and one-dimensional polyacrylamide gel electrophoresis were applied in the separation of polydeoxycytidylic acids between 3 and 18 nucleotides in chain length (dC3-dC18). In capillary zone electrophoresis (CZE), the electrophoretic behavior of homo-oligonucleotides in hydrophilic coated capillary (CEP) and polyethyleneimine coated capillary (PEI) was followed under various experimental conditions. One-dimensional polyacrylamide gel electrophoresis was chosen as a supplemental method in the characterization of electrophoretic properties of polydeoxycytidylic acids.
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