Voltametrická studie cytosinových oligonukleotidů v pufrovaném a nepufrovaném prostředí

Warning

This publication doesn't include Institute of Computer Science. It includes Faculty of Science. Official publication website can be found on muni.cz.
Title in English Voltammetric Study of Cytosine Oligonucleotides in Buffered and Non–Buffered Solutions
Authors

BALCAROVÁ Zdeňka HOLUBOVÁ Sylvie TRNKOVÁ Libuše

Year of publication 2011
Type Article in Proceedings
Conference XXXI. MODERNÍ ELEKTROCHEMICKÉ METODY
MU Faculty or unit

Faculty of Science

Citation
Field Physical chemistry and theoretical chemistry
Keywords cytosine;oligonucleotides;cyclic voltammetry;elimination voltammetry
Description The work deals with the electrochemical study of three short homo–deoxyoligonucleotides (homo–ODNs) containing cytosine (C). Using linear sweep voltammetry (LSV) and elimination voltammetry with linear scan (EVLS) the effect of pH and the number of C in the ODN chain on reduction signals of dC3, dC6, and dC9 in buffered and non-buffered solutions was investigated. Reduction C peaks, recorded at mercury electrode in the potential range from –1.1 V to –1.7 V vs. Ag/AgCl/3M KCl, are strongly dependent not only on pH but also on the composition of solutions. With the addition of buffer (acetate-phosphate buffer) to 0.1 M NaCl the reduction peaks increase and move to more positive potentials. In non-buffered solutions changes of voltammetric responses of all ODNs in time were observed. The biggest changes were recorded in the case of dC6. The adsorption or surface kinetics of ODNs was confirmed by EVLS and electrochemical behaviour of ODNs studied was discussed in the context of the i-motif formation.
Related projects:

You are running an old browser version. We recommend updating your browser to its latest version.

More info