Enantioselectivity of Haloalkane Dehalogenases and its Modulation by Surface Loop Engineering

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Authors	PROKOP Zbyněk SATO Yukari BREZOVSKÝ Jan MOZGA Tomáš CHALOUPKOVÁ Radka KOUDELÁKOVÁ Táňa JEŘÁBEK Petr ŠTĚPÁNKOVÁ Veronika NATSUME Rio LEEWEN J.G.E. JANSSEN D,B. FLORIAN J. NAGATA Yuji SENDA T. DAMBORSKÝ Jiří
Year of publication	2010
Type	Article in Periodical
Magazine / Source	Angewandte Chemie International Edition
MU Faculty or unit	Faculty of Science
Citation
web	http://loschmidt.chemi.muni.cz/peg/pdf/angewandte2010.pdf
Field	Biochemistry
Keywords	Enantioselectivity; Haloalkane Dehalogenases; protein engineering
Description	Engineering of the surface loop in haloalkane dehalogenases affects their enantiodiscrimination behavior. The temperature dependence of the enantioselectivity (lnE versus 1/T) of -bromoalkanes by haloalkane dehalogenases is reversed (red data points) by deletion of the surface loop; the selectivity switches back when an additional single-point mutation is made. This behavior is not observed for -bromoesters.
Related projects:	Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL) Center of Biocatalysis and Biotransformation Evolution of substrate specificity in enzymes acting on xenobiotic compounds