Unraveling a FNR-based regulatory circuit in bacterium Paracoccus denitrificans using proteomics, transcriptomic and bioinformatic approaches

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Authors	BOUCHAL Pavel STRUHÁROVÁ Iva BUDINSKÁ Eva ŠEDO Ondrej VYHLÍDALOVÁ Tereza ZDRÁHAL Zbyněk VAN SPANNING Rob KUČERA Igor
Year of publication	2009
Type	Conference abstract
MU Faculty or unit	Faculty of Science
Citation
Description	The switch from aerobic to anaerobic respiration in the bacterium Paracoccus denitrificans is orchestrated by the action of three Fnr-type transcription regulators FnrP, NNR and NarR, which are sensors for oxygen, nitric oxide and nitrite, respectively. In this study, we aimed to discover the role of Fnr-family transcription regulators in the aerobic-semiaerobic switch at global level. Methods and Materials: We analyzed the protein composition of four Paracoccus denitrificans strains (wild type, FnrP-, NNRand NarR- mutant strains) grown aerobically, semiaerobically and semiearobically in the presence of nitrate using two-dimensional gel electropohoresis with MS/MS protein identification, with data validation at the transcript (qRT-PCR) and genome (FNR-binding sequences) levels. Results: Expression profiles were acquired using two-dimensional gel electrophoresis for 737 protein spots, in which 640 proteins gene products were identified using mass spectrometry.
Related projects:	Proteins in metabolism and interaction of organisms with the environment The study of molecular mechanisms of bacterial adaptation to environmental changes using proteomic techniques