Phosphopeptides analysis using enrichment techniques and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

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Authors	DYČKA Filip LAŠTOVIČKOVÁ Markéta ČERNÝ Martin BRZOBOHATÝ Břetislav BOBÁLOVÁ Janette
Year of publication	2009
Type	Article in Proceedings
Conference	3rd Central and Eastern European Proteomics Conference. Book of Abstracts
MU Faculty or unit	Faculty of Science
Citation
Field	Genetics and molecular biology
Keywords	proteomic analysis; phosphoproteome
Description	In the present study, two methods for enrichment of tryptic phosphopeptides were described: IMAC and titanium dioxide chromatography. After enrichment procedure the phosphopeptides were analyzed by MALDI-TOF MS. Alpha-casein was used as model phosphoprotein. The methods were applied to protein mixture of Arabidopsis thaliana.
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