The first occurrence of the cyanobacterial alkaloid toxin cylindrospermopsin in the Czech Republic as determined by immunochemical and LC/MS methods

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Authors	BLÁHOVÁ Lucie ORAVEC Michal MARŠÁLEK Blahoslav ŠEJNOHOVÁ Lenka ŠIMEK Zdeněk BLÁHA Luděk
Year of publication	2009
Type	Article in Periodical
Magazine / Source	Toxicon
MU Faculty or unit	Faculty of Science
Citation
Field	Environment influence on health
Keywords	cyanotoxin;cylindrospermopsin;methods of detection
Description	We report comparison of the immunochemical ELISA determination with the liquid chromatography/ mass spectrometry (LC/MS) method for CYN analyses in the samples from the Czech Republic, Central Europe. Results of the ELISA and LC/MS showed good qualitative agreement. However, concentrations determined by ELISA were systematically higher than concentrations determined by LC/MS. Differences between methods could be attributed to matrix effects (both in ELISA and LC/MS) or ELISA cross-reactivity with other unidentified derivatives of CYN. Our study demonstrates for the first time occurrence of CYN in the Central Europe.
Related projects:	Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL) Tumor promotional mechanisms of cyanobacterial metabolites