Laser ablation process as the key point of GLIM

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Authors	HAVLIŠ Jan NEJEZCHLEBOVÁ Hana VACULOVIČ Tomáš KANICKÝ Viktor
Year of publication	2008
Type	Article in Proceedings
Conference	Book of abstracts XIXthe Slovak Czech Spectroscopic Conference
MU Faculty or unit	Faculty of Science
Citation
Field	Analytic chemistry
Keywords	metalloprotein;speciation;gel electrophoresis;laser ablation;ICP MS
Description	laser ablation has the key role in analysis of metalloproteins by means of GLIM. several parameters were optimised: gel form (drier dried, cellophane coating, frozen gel), laser power density and their dependence to particle number and size, and signal intensity. best parameters were found for gels frozen at -80 deg for 25 min.
Related projects:	Proteins in metabolism and interaction of organisms with the environment Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL) Molecular basis of cell and tissue regulations Development of new methods for metalloprotein characterisation