Genotyping of selected potato cultivars based on structural variation of telomere-associated chromosome regions
| Authors | |
|---|---|
| Year of publication | 2007 |
| Type | Article in Periodical |
| Magazine / Source | Vědecké práce VÚB |
| MU Faculty or unit | |
| Citation | |
| Field | Genetics and molecular biology |
| Keywords | PCR; telomere-associated sequences; potato cultivars |
| Description | Molecular genotyping techniques are of still increasing importance in agriculture practise and trade. Here we summarize results of research projects in which a novel assay for genotyping of potato cultivars was established. The assay is based on PCR using combinations of primers derived from telomeric and telomere-associated sequences. A high degree of sequence polymorphism and, namely, the variability in mutual arrangement of otherwise conserved sequence-building-blocks of subtelomeres, enabled us to establish a simple and reliable PCR-based assay to distinguish potato cultivars in 2-3 PCR reactions. |
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