Determination of D- and L-amino acids produced by cyanobacteria using gas chromatography on Chirasil-Val after derivatization with pentafluoropropyl chloroformate

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Authors	ZAHRADNÍČKOVÁ Helena HOLOUBEK Ivan
Year of publication	2007
Type	Article in Periodical
Magazine / Source	Analytical and Bioanalytical Chemistry
MU Faculty or unit	Faculty of Science
Citation
Field	Analytic chemistry
Keywords	Amino acids; cyanobacteria; gas chromatography
Description	A rapid and simple method was developed for the determination of free amino acids (AAs) released from cyanobacteria. The procedure involves trapping of AAs from the centrifuged cyanobacterial culture fluid on a cation-exchange resin, their release together with the resin by a direct treating with the reaction medium, followed by immediate derivatization with a respective chloroformate.
Related projects:	Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)