RYCHLÉ HPLC  STANOVENÍ FENOTYPU CYP 1A, 2C A 3A Z PERFUZÁTU IZOLOVANÝCH JATER POTKANA

Juřica,  Jan; Tomandl,  Josef; Dostálek,  Miroslav; McCaskey Hadašová,  Eva

RYCHLÉ HPLC STANOVENÍ FENOTYPU CYP 1A, 2C A 3A Z PERFUZÁTU IZOLOVANÝCH JATER POTKANA

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Title in English	RAPID HPLC DETERMINATION OF PHENOTYPE CYP 1A, 2C AND 3A FROM THE PERFUSATE MEDIUM AFTER ISOLATED RAT LIVER PERFUSION
Authors	JUŘICA Jan TOMANDL Josef DOSTÁLEK Miroslav HADAŠOVÁ Eva
Year of publication	2005
Type	Article in Proceedings
Conference	Sborník abstrakt 55. ČESKO-SLOVENSKÉ FARMAKOLOGICKÉ DNY
MU Faculty or unit	Faculty of Medicine
Citation
Field	Biochemistry
Keywords	phenacetine; midazolam; tolbutamide; CYP1A; CYP2C; CYP3A
Description	HPLC methods trace the markers of the metabolic activity of CYP3A1/2 (midazolam and its three hydroxymetabolites: 1-hydroxymidazolam, 1,4-dihydroxymidazolam, and 4-hydroxymidzolam), CYP2C6(tolbutamide and its metabolite hydroxytolbutamide) or CYP1A2(phenacetine and its metabolite paracetamol). Proposed methods are simple, rapid, sensitive and applicable for modeling and description of possible pharmacological interactions on rat CYP3A1/2, CYP2C6 or CYP1A2, respectively.
Related projects:	Plasticity of Regulatory Mechanisms of the Central Nervous Systems