PROTEOMIC STRATEGY FOR INVESTIGATIGATION OF CYTOKININ ACTION IN ARABIDOPSIS THALIANA

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Authors	BÖHMOVÁ Gabriela ZDRÁHAL Zbyněk KONEČNÁ Hana KOUKALOVÁ Šárka BRZOBOHATÝ Břetislav
Year of publication	2005
Type	Article in Proceedings
Conference	Molecular and cellular proteomics
MU Faculty or unit	Faculty of Science
Citation
Field	Genetics and molecular biology
Keywords	proteomics; mass spectrometry; plant hormone
Description	Proteomics-based approach for the investigation of Arabidopsis thaliana seedlings with cytokinin overproduction was applied. We used both MALDI-MS and LC-MS/MS techniques for peptide analysis. Our results showed the effective application of different methods for protein identification. In our case the combination of MALDI-MS and LC-MS/MS increased the number of identified proteins to over 90 % of all analysed samples.
Related projects:	Molecular basis of cell and tissue regulations Genomes and their functions