Action potential voltage clamp method used in analysis of rate-dependent changes of action potential

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Authors	BÉBAROVÁ Markéta MATEJOVIČ Peter PÁSEK Michal ŠIMURDOVÁ Milena ŠIMURDA Jiří
Year of publication	2004
Type	Article in Periodical
Magazine / Source	Analysis of Biomedical Signals and Images
MU Faculty or unit	Faculty of Medicine
Citation
Field	Physiology
Keywords	action potential voltage clamp; rate dependence; method; action potential
Description	Ionic currents responsible for rate-dependent changes of action potetial (AP) waveform were analyzed using AP voltage clamp method in isolated rat ventricular myocytes. The L-type calcium current ICa-L and the transient outward current Ito were found to underlie rate-dependent variability of AP duration. In addition, the fast sodium current INa played a significant role under the effect of the class I antiarrhythmic drug ajmaline, very likely as a consequence of its use-dependent block.
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