Localization of seven new late-flowering mutations on the genetic map of Arabidopsis thaliana using a newly generated CAPS marker

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Authors	LÍZAL Pavel RELICHOVÁ Jiřina
Year of publication	2004
Type	Article in Periodical
Magazine / Source	Plant Science
MU Faculty or unit	Faculty of Science
Citation
Field	Genetics and molecular biology
Keywords	Arabidopsis thaliana; DNA markers; late-flowering mutants; map position
Description	The genetic analysis of seven new late-flowering mutants is reported. One mutation was found to be recessive (dn) and six to be dominant. The relevant loci were located at distinct positions on chromosomes one and four. A new protocol for converting RFLP (Restriction Fragment Length Polymorphism) markers to CAPS (Cleaved Amplified Polymorphic Sequences) as PCR-based markers was developed using the Arabidopsis thaliana genome database. RFLP marker MI122 was successfully converted to a CAPS, which allowed more precise mapping of five late-flowering mutations.
Related projects:	The interaction of the late flowering genes in Arabidopsis thaliana and their localization on the genetic map Genomes and their functions