Automated micro axial tomography of cell nuclei after specific labelling by fluorescence in situ hybridisation
| Authors | |
|---|---|
| Year of publication | 2002 |
| Type | Article in Periodical |
| Magazine / Source | Micron |
| MU Faculty or unit | |
| Citation | |
| Field | Use of computers, robotics and its application |
| Keywords | micro axial tomography; automated microscopy; high-resolution cytometry; FISH imaging; interphase cell nuclei |
| Description | Micro axial tomography provides a good means for microscopic image acquisition of cells or sub-cellular components like cell nuclei with an improved resolution, because shortcomings of spatial resolution anisotropy in optical microscopy can be overcome. Thus, spatial information of the object can be obtained without the necessity of confocal imaging. Since the very early developments of micro axial tomography a considerable drawback of this method was a complicated image acquisition and processing procedure that requires much operator time. In order to solve this problem the Heidelberg 2pi-tilting device has been mounted to the Brno high-resolution cytometer as an attempt to bring together advanced microscopy and fast automated computer image acquisition and analysis. A special software module that drives all hardware components required for automated micro axial tomography and performs image acquisition and processing has been developed. Also a method for the preparation of cell nuclei attached to glass fibres has been developed that allows for the first time imaging of three-dimensionally conserved, FISH-stained cell nuclei fixed to a glass fibre. |
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