Interakce iontů kovů s imobilizovanou DNázou I

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Title in English	Interactions of metal ions with immobilized DNase I
Authors	FALK Martin ŠPANOVÁ Alena RITTICH Bohuslav
Year of publication	2002
Type	Article in Proceedings
Conference	VI. Pracovní setkání biochemiků a molekulárních biologů
MU Faculty or unit	Faculty of Science
Citation
Field	Microbiology, virology
Keywords	DNA cleavage; DNase I; immobilization; activating ions; lanthanides
Description	DNaseI cleaves both strands od DNA molecule by chance in the presence of Mg2+ and Ca2+ ions. DNaseI immobilised on magnetic cellulose was choosed as a model system. Lanthanides have the same chemical properties as Ca2+ ions. Lanthanides have an inhibitory effect on enzyme activity activated with divalent cations in many cases.Cleavage of chromosomal DNA was not detected using DNaseI activated with La3+ ions. Cleavage of plasmid DNA was detected in the presence of some lanthanides ions only.
Related projects:	The use of magnetic carriers with immobilized selective ligands in DNA diagnostics