CRISPR-ASSISTED STRUCTURAL MODIFICATION OF STAPHYLOCOCCAL BACTERIOPHAGES FOR IMAGING AND BIOSENSING APPLICATIONS
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| Year of publication | 2025 |
| Type | Conference abstract |
| MU Faculty or unit | |
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| Description | Lytic bacteriophages are effective therapeutic agents against antibiotic antibiotic-resistant bacteria. Furthermore, recent advances in CRISPR CRISPR-CasCas–based genome editing techniques have expanded the possibilities of engineering phages with novel and unique properties. In our study, the polyvalent Staphylococcus aureus phage 812h1 was genetically modified by inserting a polyhistidine tag into an exposed loop of the tail sheath protein. An editing strategy combining homologous recombination with CRISPR CRISPR-Cas10Cas10-assisted countercounter-selection allowed the construction of stable recombinant particles. The His His-phages were specifically recognized by antibodies, and their attachment to bacteria was visualized by fluorescence microscopy. The structural modifications did not impair the biological activity of the phages, and their functionality was further validated using bio bio-layer interferometry, enzyme enzyme-linked immunosorbent assay, and flow cytometry 1 . The engineered phages provide new opportunities for applications in research, diagnostics, environmental monitoring, and the development of advanced biosensing tools. |
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