Ozonization for MALDI-2 MS imaging of carbon–carbon double bond positional isomers of phosphatidylethanolamines in biological tissues

Warning

This publication doesn't include Institute of Computer Science. It includes Faculty of Science. Official publication website can be found on muni.cz.

Authors	BEZDEKOVÁ Dominika HENDRYCH Michal SCHWENZFEIER Jan SOLTWISCH Jens DREISEWERD Klaus VLČEK Petr PREISLER Jan BEDNAŘÍK Antonín
Year of publication	2025
Type	Article in Periodical
Magazine / Source	Analytica Chimica Acta
MU Faculty or unit	Faculty of Science
Citation
web	https://www.sciencedirect.com/science/article/pii/S0003267025012085
Doi	https://doi.org/10.1016/j.aca.2025.344814
Keywords	on-tissue reaction; ozonization; MALDI MSI; MALDI-2; whole-body imaging; colon adenocarcinoma; lipid isomers
Description	This study demonstrates a coupling of on-tissue ozonization with MALDI-2 mass spectrometry imaging for mapping of phosphatidylethanolamine (PE) isomers in biological tissues. Whole-body section imaging of mice revealed distinct distributions of lipid isomers across organs, notably identifying brown adipose tissue as an interesting target for isomer analysis. Mapping of PE isomers in human low-grade colon adenocarcinoma demonstrates the potential of the technique for studying lipid metabolism in tumors.
Related projects:	Molecular Mechanisms and Biomarkers: Unraveling Complex Challenges in Neuro-Oncology Digital multiplex mass spectrometry imaging Pathology of Tumorous and Non-Tumorous Diseases