Fágový trénink a izolace spontánních mutant jako cesta ke zvýšení účinnosti terapeutických fágů proti klinickým kmenům Staphylococcus aureus
| Title in English | Phage training and isolation of spontaneous mutants as a way to increase the efficacy of therapeutic phages against clinical strains of Staphylococcus aureus |
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| Authors | |
| Year of publication | 2025 |
| Type | Conference abstract |
| MU Faculty or unit | |
| Citation | |
| Description | Chronic infections caused by Staphylococcus aureus pose a significant challenge, as infected bacterial cells exhibit reduced metabolic activity and consequently lower sensitivity to antibiotics and phages. This work aimed to obtain phages capable of effectively infecting and lysing bacteria in the stationary phase. To this end, spontaneous phage mutants were isolated, and phage training was used. Phage 812vo was obtained by isolating plaques of spontaneous mutants of phage 812 on the resistant strain S. aureus 11/608, and its activity was tested on exponential and stationary cultures. The sequence of its genome was determined and compared with the parental phage. Genomic analysis of phage 812vo revealed the presence of several mutations in genes encoding hypothetical proteins. Phage 812vo exhibited higher lytic activity during the exponential phase compared to the original phage and was able to infect the originally resistant strain effectively. In addition, it was able to lyse cells in the stationary phase. Phage P68 underwent ten days of training on stationary cultures of S. aureus strain 11/608 in three different culture media, followed by testing of lytic activity upon infection of exponential and stationary cultures. The trained phage P68 showed different lytic activity depending on the culture medium used. In some cases, improved efficacy was observed when infecting stationary cultures. The results show that adaptation and phage training can increase the effectiveness of phages against S. aureus populations in various physiological states and represent a promising avenue for expanding the possibilities of phage therapy for chronic infections. |
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