Vliv koevolučního fágového tréninku v přítomnosti antibiotik na fenotyp a genotyp kmenů Staphylococcus aureus
| Title in English | The effect of coevolutionary phage training in the presence of antibiotics on the phenotype and genotype of Staphylococcus aureus strains |
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| Year of publication | 2025 |
| Type | Conference abstract |
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| Description | The spread of multidrug-resistant strains of bacterial pathogens has a negative impact on the course and treatment of the diseases they cause. An important aspect in addressing this problem is not only understanding the mechanisms of resistance development, but also finding complementary or alternative treatments to standard antibiotics. This is phage therapy using bacterial viruses (bacteriophages), possibly in combination with antibiotics. Its advantage is the ability of phages to adapt and infect previously resistant strains, which can be achieved in a targeted and effective manner in a laboratory environment through adaptive laboratory evolution (ALE), known as phage training. In the presented work, ALE was used for co-evolutionary phage training, in which the evolutionary adaptation of the staphylococcal lytic phage of the genus Kayvirus and the bacterial strain occurs simultaneously, in the presence and absence of the antibiotic amoxicillin (AML). The experiment aimed to evaluate how the selection pressure of selected phages, AML, or a combination of phages and AML affects the evolutionary trajectory of the clinical strain of Staphylococcus aureus in laboratory conditions. The experiment was performed using 10 passages, and the obtained variants of the clinical strain were subsequently characterized using an antibiotic disc diffusion test, spectrophotometry, and whole-genome sequencing on the Oxford Nanopore platform. An increased incidence of antibiotic resistance was observed in variants exposed to antibiotics. At the same time, resistance to the phages used either developed or was lost. The results also showed that the combination of phages and AML did not lead to a significantly different development of resistance compared to the effect of AML alone. |
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