Isolation of Compounds with Potential Anti-inflammatory Activity from Glycyrrhiza uralensis via Bioassay-Guided Fractionation
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| Year of publication | 2025 |
| Type | Appeared in Conference without Proceedings |
| MU Faculty or unit | |
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| Description | Glycyrrhiza species have been used worldwide in traditional medicine for their expectorant, anti-inflammatory, and antimicrobial activities. The anti-inflammatory effects are attributed primarily to prenylated phenolics, which exhibit unique interactions with biological membranes, making them promising candidates for drug development. The main objective of this study was the isolation of prenylated phenolics with potential anti-inflammatory activity from Glycyrrhiza uralensis. The chloroform fraction from G. uralensis was selected, as its extracts demonstrated the highest bioactivity in our previous study. The plant material was obtained through cooperation with the Faculty of Horticulture, Mendel University. An ethanolic extract from freshly harvested roots was subjected to liquid-liquid partitioning with n-hexanes, chloroform, ethyl acetate, and water, respectively. The chloroform fraction was chromatographed on RP-C18, yielding fifteen unique fractions. These fractions were evaluated for cytotoxicity and subjected to biological testing for NF-?B inhibition using the Quanti-Blue™ assay on THP-1 cells. Compounds from fractions exhibiting the highest anti-inflammatory activity were isolated by semi-preparative HPLC. The structures of the isolated compounds were elucidated by 2D NMR spectroscopy. In conclusion, we successfully isolated and identified a total of nine prenylated isoflavonoids from fractions exerting the most prominent bioactivities, which will undergo further biological testing. |
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