Upravená dehalogenáza LinB116 se zlepšenou termostabilitou
| Title in English | Engineered dehalogenase LinB116 with improved thermostability |
|---|---|
| Authors | |
| Year of publication | 2024 |
| Type | Outcomes put into operation (prototype, working sample) |
| MU Faculty or unit | |
| web | https://loschmidt.chemi.muni.cz/peg/ |
| Description | The wild-type haloalkane halogenase LinB represents an attractive enzyme with high application potential due to its ability to degrade cyclic halogenated hydrocarbons. Using protein engineering methods, a thermostable variant of LinB116 carrying a total of 9 stabilising mutations was designed. Due to increasing the melting temperature by more than 15 °C to almost 65 °C, LinB116 can be more efficiently used even under conditions where wild-type LinB already loses its functionality. |
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