Sanger sequencing of mitochondrial hypervariable region of ancient samples for DNA authentication and screening before high-throughput sequencing

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Authors

ŠENOVSKÁ Anna DROZDOVÁ Eva BRZOBOHATÁ Kristýna CHOCHOLOVÁ Eva FIALOVÁ Dana ŠMERDA Jaromír

Year of publication 2021
Type Article in Periodical
Magazine / Source Journal of Archaeological Science: Reports
MU Faculty or unit

Faculty of Science

Citation
Web https://doi.org/10.1016/j.jasrep.2021.103216
Doi http://dx.doi.org/10.1016/j.jasrep.2021.103216
Keywords ancient DNA; mitochondrial DNA; Sanger sequencing; hypervariable region
Description Working with ancient DNA is challenging and requires an authentication step. We analyzed eleven badly preserved samples from the Nitra culture with the influence of Únětice culture dated to the 4000 BP Bronze Age. We present estimated mitochondrial haplogroups of four samples based on Sanger sequencing of HVR1 and a part of HVR2. Sanger sequencing has strong limitations in haplogroup prediction, especially when working with ancient DNA. However, Sanger sequencing in this study is featured as a control step to provide us with quality results of mitochondrial DNA and as a screening of present haplogroups. DNA quality estimation is further supported by sample concentration and fragment analysis. The presence of mitochondrial DNA is confirmed by PCR. However, the Sanger sequencing of mitochondrial DNA can confirm its presence in good quality for further sequencing. Therefore, only suitable samples were selected for further high-throughput sequencing of the whole mitogenome.
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