Surface-enhanced Raman Spectroscopy in Microfluidic Chips for Directed Evolution of Enzymes and Environmental Monitoring

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Authors

PILAT Zdenek JEZEK Jan KIZOVSKY Martin KLEMENTOVA Tereza KRATKY Stanislav SOBOTA Jaroslav SAMEK Ota ZEMANEK Pavel BURYŠKA Tomáš DAMBORSKÝ Jiří PROKOP Zbyněk

Year of publication 2019
Type Article in Proceedings
Conference 2019 PHOTONICS & ELECTROMAGNETICS RESEARCH SYMPOSIUM - SPRING (PIERS-SPRING)
MU Faculty or unit

Faculty of Science

Citation
Web https://ieeexplore.ieee.org/document/9017521
Doi http://dx.doi.org/10.1109/PIERS-Spring46901.2019.9017521
Keywords Raman Spectroscopy; 4-Aminothiophenol; Plasmonics
Description Optofluidics, a research discipline combining optics with microfluidics, currently aspires to revolutionize the analysis of biological and chemical samples e.g. for medicine, pharmacology, or molecular biology. In order to detect low concentrations of analytes in water, we developed an optofluidic device containing a nanostructured substrate for surface enhanced Raman spectroscopy (SERS). The geometry of the gold surface allows localized plasmon oscillations to give rise to the SERS effect, in which the Raman spectral lines are intensified by the interaction of the plasmonic field with the electrons in the molecular bonds. The SERS substrate was enclosed in a microfluidic system, which allowed transport and precise mixing of the analyzed fluids, while preventing contamination or abrasion of the highly sensitive substrate. To illustrate its practical use, we employed the device for quantitative detection of persistent environmental pollutant 1,2,3-trichloropropane in water in millimolar and even submillimolar concentrations. The developed sensor allows fast and simple quantification of halogenated compounds and it will contribute towards the environmental monitoring and enzymology experiments with engineered haloalkane dehalogenase enzymes.
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