Blocking the Nanopores in a Layer of Nonconductive Nanoparticles: Dominant Effects Therein and Challenges for Electrochemical Impedimetric Biosensing

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This publication doesn't include Institute of Computer Science. It includes Central European Institute of Technology. Official publication website can be found on muni.cz.
Authors

SOPOUŠEK Jakub VĚŽNÍK Jakub SKLÁDAL Petr LACINA Karel

Year of publication 2020
Type Article in Periodical
Magazine / Source ACS Applied Materials & Interfaces
MU Faculty or unit

Central European Institute of Technology

Citation
Web https://doi.org/10.1021/acsami.0c02650
Doi http://dx.doi.org/10.1021/acsami.0c02650
Keywords nanopores; biosensing; electrochemical impedance spectroscopy; polystyrene nanoparticles; influence of charge
Description Blockage of a nanopore by an analyte molecule has emerged as a promising concept for electrochemical biosensing. Nanoporous structures can be formed on the electrode surface simply by packing spherical nanoparticles in a dense planar arrangement. Modification of the nanoparticles with human serum albumin (HSA) and its interaction with the corresponding antibody (anti-HSA) can induce nanopore-blockage which significantly hinders permeation of the redox probe ([Fe(CN6)](4-/3-)). Interfaces of different parameters were studied using Electrochemical Impedance Spectroscopy (EIS), and counterintuitively, the influence of charge of the nanoparticles and other immobilized entities played a substantial role in the measurement. Our study reveals dominant effects including the presence of mixed output signal and resolves corresponding EIS biosensing-related challenges. Consequently, blocking the nanopores was introduced as an efficient technique which enables the application of EIS-based biosensing to real-world analytical issues.
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