Drug Penetration Analysis in 3D Cell Cultures Using Fiducial-Based Semiautomatic Coregistration of MALDI MSI and Immunofluorescence Images

Investor logo

Warning

This publication doesn't include Institute of Computer Science. It includes Faculty of Science. Official publication website can be found on muni.cz.
Authors

MACHÁLKOVÁ Markéta PAVLATOVSKÁ Barbora MICHÁLEK Jan PRUŠKA Adam ŠTĚPKA Karel NEČASOVÁ Tereza RADASZKIEWICZ Katarzyna Anna KOZUBEK Michal ŠMARDA Jan PREISLER Jan NAVRÁTILOVÁ Jarmila

Year of publication 2019
Type Article in Periodical
Magazine / Source Analytical Chemistry
MU Faculty or unit

Faculty of Science

Citation
Web https://pubs.acs.org/doi/10.1021/acs.analchem.9b02462
Doi http://dx.doi.org/10.1021/acs.analchem.9b02462
Keywords MALDI MSI; LSCM; IHC; multimodal imaging; spheroids; coregistration; image analysis
Description In this paper, we present an easy-to-follow procedure for the analysis of tissue sections from 3D cell cultures (spheroids) by matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) and laser scanning confocal microscopy (LSCM). MALDI MSI was chosen to detect the distribution of the drug of interest, while fluorescence immunohistochemistry (IHC) followed by LSCM was used to localize the cells featuring specific markers of viability, proliferation, apoptosis and metastasis. The overlay of the mass spectrometry (MS) and IHC spheroid images, typically without any morphological features, required fiducial-based coregistration. The MALDI MSI protocol was optimized in terms of fiducial composition and antigen epitope preservation to allow MALDI MSI to be performed and directly followed by IHC analysis on exactly the same spheroid section. Once MS and IHC images were coregistered, the quantification of the MS and IHC signals was performed by an algorithm evaluating signal intensities along equidistant layers from the spheroid boundary to its center. This accurate colocalization of MS and IHC signals showed limited penetration of the clinically tested drug perifosine into spheroids during a 24 h period, revealing the fraction of proliferating and promigratory/proinvasive cells present in the perifosine-free areas, decrease of their abundance in the perifosine-positive regions and distinguishing between apoptosis resulting from hypoxia/nutrient deprivation and drug exposure.
Related projects:

You are running an old browser version. We recommend updating your browser to its latest version.

More info