Targeted-random mutagenesis of PA-IIL lectin and high-throughput screening of mutant library

Warning

This publication doesn't include Institute of Computer Science. It includes Faculty of Science. Official publication website can be found on muni.cz.

Authors	MRÁZKOVÁ Jana POKORNÁ Martina WIMMEROVÁ Michaela
Year of publication	2014
Type	Conference abstract
MU Faculty or unit	Faculty of Science
Citation
Description	PA-IIL lectin from Pseudomonas aeruginosa shows a unique binding mode among bacterial lectins because it binds saccharides through two calcium cations. Its features make this protein a good candidate for tuning its affinity and specificity. Two amino acids responsible for sugar binding were chosen for targeted-random mutagenesis. If this approach is used, it is necessary to utilize high-throughput screening of mutant libraries for identification of mutants with interestingly modified specificity and/or affinity.
Related projects:	CEITEC - Central European Institute of Technology Studium proteinu z patogenu zapojených do rozpoznávání hostitelského organismu