A simple Fourier filter for suppression of the missing wedge ray artefacts in single-axis electron tomographic reconstructions

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Authors

KOVÁČIK Lubomír KEREICHE Sami HÖÖG Johanna JŮDA Pavel MATULA Pavel RAŠKA Ivan

Year of publication 2014
Type Article in Periodical
Magazine / Source Journal of Structural Biology
MU Faculty or unit

Faculty of Informatics

Citation
Doi http://dx.doi.org/10.1016/j.jsb.2014.02.004
Field Genetics and molecular biology
Keywords Electron tomography; Missing wedge; Missing wedge artefacts; Single-axis tilting
Description he limited specimen tilting range that is typically available in electron tomography gives rise to a region in the Fourier space of the reconstructed object where experimental data are unavailable - the missing wedge. Since this region is sharply delimited from the area of available data, the reconstructed signal is typically hampered by convolution with its impulse response, which gives rise to the well-known missing wedge artefacts in 3D reconstructions. Despite the recent progress in the field of reconstruction and regularization techniques, the missing wedge artefacts remain untreated in most current reconstruction workflows in structural biology. Therefore we have designed a simple Fourier angular filter that effectively suppresses the ray artefacts in the single-axis tilting projection acquisition scheme, making single-axis tomographic reconstructions easier to interpret in particular at low signal-to-noise ratio in acquired projections. The proposed filter can be easily incorporated into current electron tomographic reconstruction schemes.
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