Crystallization and preliminary X-ray crystallographic analysis of recombinant beta-mannosidase from Aspergillus niger

Warning

This publication doesn't include Institute of Computer Science. It includes Central European Institute of Technology. Official publication website can be found on muni.cz.

Authors	DEMO Gabriel FLIEDROVA Barbora WEIGNEROVA Lenka WIMMEROVÁ Michaela
Year of publication	2013
Type	Article in Periodical
Magazine / Source	Acta crystallographica. Section F
MU Faculty or unit	Central European Institute of Technology
Citation
Doi	https://doi.org/10.1107/S1744309113002522
Field	Biochemistry
Keywords	Mannosidase; Aspergillus niger; Expression; Crystallization
Description	beta-Mannosidase (EC 3.2.1.25) is an important exoglycosidase specific for the hydrolysis of terminal beta-linked mannoside in various oligomeric saccharide structures. beta-Mannosidase from Asperigillus niger was expressed in Pichia pastoris and purified to clear homogeneity. beta-mannosidase was crystallized in the presence of D-mannose and the crystal diffracted to 2.41 A resolution. The crystal belonged to space group P1. Monomeric structure was observed in the asymmetric unit.
Related projects:	Enzymy metabolismu mannosidů v přípravě N-acetyl-mannosaminových struktur CEITEC - Central European Institute of Technology