Crystallization and preliminary X-ray crystallographic analysis of beta-D-mannosidase from Asperigillus niger

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Authors	DEMO Gabriel FRIEDLOVA Barbora WEIGNEROVA Lenka WIMMEROVÁ Michaela
Year of publication	2012
Type	Conference abstract
MU Faculty or unit	Central European Institute of Technology
Citation
Description	Mannosidases are important enzymes involved in the glycosidic bond hydrolysis and cleavage of beta-D-mannose from oligomeric saccharides. Fungal beta-D-mannosidase was crystallized in the presence of D-mannose and iodine compound. Diffraction data were collected at HZB-BESSY on beamline 14.1. Detailed structural information will help to understand its functional role and the conformational behaviour of the substrate in the retaining glycosylhydrolase mechanism.
Related projects:	Enzymy metabolismu mannosidů v přípravě N-acetyl-mannosaminových struktur