Expression of hypothetical lectin from pisum sativum-comparison of two eukaryotic expression systems

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Authors	DEJMKOVÁ Eva ŠEBELA Marek WIMMEROVÁ Michaela
Year of publication	2012
Type	Conference abstract
MU Faculty or unit	Faculty of Science
Citation
Description	We have identified a new putative lectin from Pisum sativum using SDS-PAGE and MS analysis of highly purified pea amine oxidase where it was present as an impurity. This study was aimed at the production of this protein in a eucaryotic expression system and its structure-function characterization. As production of the native protein was found very low, we have compared two yeast expression systems - Pichia pastoris and Saccharomyces cerevisiae. Advantages/disadvantages of both approaches will be discussed in detail.
Related projects:	Structure-functional characterization of oxidoreductases acting on nitrogenous regulatory compounds in plants CEITEC - Central European Institute of Technology