Project information
Investigation of the local control of gene expression using spectral microscopy and omage analysis techniques
- Project Identification
- GA301/01/0186
- Project Period
- 1/2001 - 1/2003
- Investor / Pogramme / Project type
-
Czech Science Foundation
- Standard Projects
- MU Faculty or unit
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Faculty of Informatics
- RNDr. Petr Mejzlík, Dr.
- Cooperating Organization
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Institute of Biophysics
- Responsible person doc. RNDr. Stanislav Kozubek, DrSc.
Using the high-resolution cytometry, we have established the positions of some genes, centromeres and chromosomes in nuclei of different cell types and their changes during basic cellular processes such as cell cycle, differentiation, repair of damage, e tc. We have also found that the changes in the gene location may correlate with gene silencing during differentiation, which strongly suggest that chromatin structure represents an important factor in the regulation of gene expression. in this project, t he local structure in the close neighbourhood of genes will be studied and correlation to gene expression will be investigated. We have already developed the spectral microscopy (SM) technique for these purposes which allows us to detect distances below 50 nm. As a model example, the globin locus will be analysed: the DNA probes will be prepared for the local control region (LCR) as well as for the beta-globin region (labelled with different fluorochrome) on the opposite sides of the locus and their dis
Publications
Total number of publications: 15
2004
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Topographic characterization of telomeres in healthy and various tumor cell lines determined in situ and in vivo
Year: 2004, type: Conference abstract
2003
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Architecture of chromosomal territories in apoptotic cells
Biophysics of the Genome, year: 2003
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Arrangement of chromosome 11 and 22 territories, EWSR1 and FLI-1 genes, and other genetic elements of these chromosomes in human lymphocytes and Ewing sarcoma cells
Human Genetics, year: 2003, volume: 112, edition: 2
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Cytogenetics and cytology of retinoblastomas
J Cancer Res. Clin.Oncol., year: 2003, volume: 129, edition: 2
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Chromosomal territory segmentation in apoptotic cells
Cellular and Molecular Life Sciences, year: 2003, volume: 60, edition: 5
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Nuclear and territorial topography of chromosome telomeres in human lymphocytes
Experimental Cell Research, year: 2003, volume: 289, edition: 1
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Topography of telomeres and structure of chromosome territories in human lymphocytes
Biophysics of the Genome, year: 2003
2002
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3D Structure of the human genome: Order in randomness
Chromosoma, year: 2002, volume: 111, edition: 5
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Nuclear structure and gene activity in human differentiated cells
Journal of Structural Biology, year: 2002, volume: 139, edition: 2
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Optimisation of FISH Methodology for the Study of 3D Structure of Cell Nuclei Using Micro Axial Tomography
Biophysics of the Genome and Its Interactions, year: 2002