Protective role of salbutamol against aminophylline-induced arrhythmias in an hPSC-based cardiac model
| Autoři | |
|---|---|
| Rok publikování | 2025 |
| Druh | Stať ve sborníku |
| Konference | EUROPEAN RESPIRATORY JOURNAL |
| Fakulta / Pracoviště MU | |
| Citace | |
| www | https://publications.ersnet.org/content/erj/66/suppl69/pa1410 |
| Doi | https://doi.org/10.1183/13993003.congress-2025.PA1410 |
| Klíčová slova | Cellular biosensor; atomic force microscopy; arrhythmia; cardiomyocytes; drug screening; drug cardiotoxicity; conduction; pluripotent stem cells; biomechanical properties. |
| Popis | Introduction: Aminophylline and salbutamol are commonly used in clinical practice to treat obstructive lung diseases. While the adverse effects of individual bronchodilators, including arrhythmias, are well-documented, the potential side effects of their combined use remain largely unknown. Aims: This study aimed to evaluate the arrhythmogenic potential of aminophylline and salbutamol co-administration in vitro. Methods: We used an established atomic force microscopy (AFM) model integrated with cardiac organoids derived from human pluripotent stem cells (hPSC-CMs). The study focused on assessment of chronotropic, inotropic, and arrhythmogenic effects of salbutamol alone and in combination with aminophylline. To detect arrhythmic events, we employed heart rate/beat rate variability (HRV/BRV) analysis in AFM recordings from hPSC-CMs. Results: Salbutamol reduced aminophylline-induced arrhythmogenicity, likely through endothelial nitric oxide synthase activation via beta-2 adrenergic receptors. These findings were validated using hPSC-CMs derived from two distinct cell lines (CCTL4 and CCTL12). Conclusion: Our results suggest that salbutamol, beyond its bronchodilator properties, may increase the cardiovascular safety of aminophylline by reducing its arrhythmogenic potential. |
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